Name: Capillary Electrophoresis of Nucleic Acids (en Inglés)
Price: 318.77 PEN
Availability: InStock
Author: Mitchelson, Keith R. ; Cheng, Jing
ISBN: 9781617371776

portada Capillary Electrophoresis of Nucleic Acids (en Inglés)

Capillary Electrophoresis of Nucleic Acids (en Inglés)

Formato

Libro Físico

Editorial

Humana

Autor

Mitchelson, Keith R. ; Cheng, Jing

Idioma

Inglés

N° páginas

408

Encuadernación

Tapa Blanda

ISBN

1617371777

ISBN13

9781617371776

Categorías

Genética médica
Bioquímica

Capillary Electrophoresis of Nucleic Acids (en Inglés)

Mitchelson, Keith R. ; Cheng, Jing (Autor) · Humana · Tapa Blanda

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Reseña del libro "Capillary Electrophoresis of Nucleic Acids (en Inglés)"

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single-strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.